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Answer :
The Entner-Doudoroff (ED) pathway being the primary metabolism route in certain ancient bacteria and its lower ATP yield compared to the EMP pathway suggest it may be the more primitive form of glucose metabolism. Therefore option 4) is correct answer.
Evidence that the Entner-Doudoroff (ED) pathway might have evolved earlier than the Embden-Meyerhof-Parnas (EMP) pathway includes the fact that the ED pathway is the primary pathway for glucose metabolism in certain bacteria. While the EMP pathway is common in both animals and many microbes, the presence of the ED pathway in some bacteria, especially in ones that may represent a more ancient lineage, like Pseudomonas aeruginosa, suggests it might be the older route. Additionally, the ED route produces fewer ATP molecules compared to the EMP pathway, which might imply it is a more primitive form of glucose metabolism because it's less efficient in energy production.
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