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You have a bacterial strain with a mutation that removes the transcription termination signal from the Abd operon. Which of the following statements describes the most likely effect of this mutation on Abd transcription?

A. The Abd RNA will not be produced in the mutant strain.
B. The Abd RNA from the mutant strain will be longer than normal.
C. Sigma factor will not dissociate from RNA polymerase when the Abd operon is being transcribed in the mutant strain.
D. RNA polymerase will move in a backward fashion at the Abd operon in the mutant strain.

Answer :

Final answer:

If the transcription termination signal from the Abd operon is removed due to a mutation, the transcription may continue past its normal stopping point, leading to a longer than normal Abd RNA transcript.

Explanation:

In the given scenario where a bacterial strain has a mutation that removes the transcription termination signal from the Abd operon, the most likely effect of this mutation on Abd transcription is that the Abd RNA from the mutant strain will be longer than normal. This is because the termination signal plays a crucial role in stopping the process of transcription. If this signal is removed due to a mutation, the transcription may continue past its usual stopping point, leading to a longer RNA transcript.

Usually, once a gene is transcribed, the prokaryotic polymerase needs a termination signal to stop the process. There are two types of termination signals in prokaryotic transcription: Rho-dependent and Rho-independent. Both work in different ways to stall the progress of RNA polymerase and release the newly formed RNA transcript.

Without the termination signal in the Abd operon, the process of transcription will not be prompted to stop. As a result, the RNA transcript will continue to be extended, forming a longer than normal RNA transcript.

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