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Answer :
Final answer:
Steam sterilization typically occurs at 250-270 DegreesF in an autoclave to ensure complete sterilization, including the destruction of endospores. Option C.
Explanation:
Steam sterilization is performed at 250-270 DegreesF (121-132 DegreesC), depending on the items being sterilized. This moist-heat sterilization protocol involves steam under pressure in an autoclave, allowing the steam to reach temperatures higher than the boiling point of water. Autoclaves function typically at a pressure of 15 to 20 pounds per square inch (psi) and require a minimum exposure of 20 minutes to effectively kill all microorganisms, including endospores which are known to survive boiling temperatures.
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