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The ______ ______ technique is an agar diffusion test to determine antibiotic susceptibility of a particular bacterial strain.

Answer :

Final answer:

The Kirby-Bauer disk diffusion technique is a method used to determine the antibiotic susceptibility of different bacterial strains. The process involves the use of an agar plate and susceptible bacteria, on which drug-soaked disks are placed. The extent of bacterial growth inhibition is a direct representation of the antibiotic's effectiveness.

Explanation:

The blank spaces in your question are filled by the term 'Kirby-Bauer disk diffusion'. This technique helps in {determining the antibiotic susceptibility of a specific bacterial strain}. The process includes placing drug-impregnated discs on an agar plate already inoculated with a particular bacterial strain. As the bacteria grow, the antibiotics present in the disks diffuse across the agar and interact with the growing bacteria. The resistance or susceptibility of the bacteria to the antibiotics is observed in the form of a clear circular zone around the disc. The greater the diameter of this zone, the higher the susceptibility of the bacteria to the antibiotic.

This method, known as antimicrobial susceptibility testing (AST), is commonly performed in clinical laboratories to understand the effectiveness of antibiotics on specific organisms. Although this method gives us limited information, it is a critical starting point for the determination of effective antibiotics for different bacterial strains.

There are alternative methods such as the Etest and the Dilution Tests which provide us with a more comprehensive understanding, including both the MIC (Minimal Inhibitory Concentration) and MBC (Minimal Bactericidal Concentration), of the antibiotic's effectiveness.

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