High School

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1. You study three different Hfr bacterial strains and analyze the order in which they transfer chromosomal genes by conjugation by disrupting the conjugation at various time points. You find the following:

1a. Draw a map of the complete bacterial chromosome. (Hint: there are seven genes on the circular chromosome.)

1b. In strain #2, between which two genes has the F-plasmid integrated?

Answer :

Final answer:

To draw a map of the complete bacterial chromosome, consider the order of gene transfer during conjugation. In strain #2, the F-plasmid has integrated between two specific genes. Analyzing the transfer patterns can help identify these genes.

Explanation:

To draw a map of the complete bacterial chromosome, we need to consider the order in which the genes are transferred during conjugation. Since there are seven genes on the circular chromosome, we can represent them as follows:

  1. Gene A
  2. Gene B
  3. Gene C
  4. Gene D
  5. Gene E
  6. Gene F
  7. Gene G

Now, let's move on to the second part of the question. In strain #2, we need to identify the two genes between which the F-plasmid has integrated. To determine this, we can analyze the transfer patterns of the genes during conjugation experiments. By disrupting the conjugation at various time points and observing the order in which the genes are transferred, we can identify the location of the F-plasmid integration.

Learn more about genes with f-plasmid integration here:

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Rewritten by : Barada

1. The map of the complete bacterial chromosome for the three Hfr strains:

  • Genes: A B C D E F G
  • Strain 1: A B C D E F
  • Strain 2: A B C D E F G
  • Strain 3: A B C D E F G

2. In strain #2, the F-plasmid has integrated between the genes D and E.

Strain 1: This strain transfers all of the genes on the chromosome, so the F-plasmid must be integrated somewhere at the end of the chromosome.

Strain 2: This strain transfers the genes A through F, but not G. This means that the F-plasmid must be integrated between the genes E and F.

Strain 3: This strain transfers the genes A through D, but not E or F. This means that the F-plasmid must be integrated between the genes D and E.

Therefore, the only place that the F-plasmid could be integrated in strain #2 is between the genes D and E.

For such more question on chromosome:

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