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The human hexokinase enzyme has the same function as the bacterial hexokinase enzyme but differs slightly in its amino acid sequence. You have obtained a mutant bacterial strain in which the gene for hexokinase and its promoter are missing. If you introduce into your mutant strain a DNA plasmid engineered to contain the coding sequence of the human hexokinase gene, driven by the normal bacterial promoter, what will the resulting bacteria produce?

Answer :

The resulting bacteria will produce a functional human hexokinase enzyme, which can catalyze the same biochemical reaction as the bacterial hexokinase enzyme. This is because the function of hexokinase is conserved across species, despite differences in amino acid sequence.

Hexokinase is an enzyme that catalyzes the conversion of glucose to glucose-6-phosphate, an important step in glucose metabolism. The basic function of hexokinase is similar in both humans and bacteria, despite differences in amino acid sequence.

The use of a bacterial promoter to drive expression of the human hexokinase gene in the mutant bacterial strain allows for the production of a functional human hexokinase enzyme, which can carry out the necessary biochemical reaction.

This technique of using a heterologous gene to replace a missing or non-functional gene is a common tool in molecular biology and biotechnology.

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