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True or False: A bacterial strain that is pro+ thi+ leu- met- will grow on minimal media plus leucine and thiamine.

Answer :

Final answer:

A bacterial strain with the pro+ thi+ leu- met- genotype will be able to grow on minimal media supplemented with leucine and thiamine. Though it can produce thiamine on its own and receives leucine from the media, it requires methionine from an external source for optimal growth which is not provided. The statement is true.

Explanation:

True. The bacterial strain in the question has the genotype pro+ thi+ leu- met-. In genetic notation, '+' indicates that the bacterium can make that substance on its own and '-' indicates it requires an external source of that substance to grow. Therefore, this strain can make proline (pro+) and thiamine (thi+) but needs leucine (leu-) and methionine (met-) from an outside source.

The question asks whether this strain can grow on minimal media supplemented with leucine and thiamine. Because the bacterium can make thiamine on its own (thi+), even though the media provides it, it still needs leucine (leu-) which is provided, and it can grow. However, for complete growth on minimal media, it also requires methionine (met-), which is not provided. This information suggests that the bacteria would be able to grow, but not completely efficiently in terms of its maximum potential due to the lack of methionine.

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