DNA from a bacterial strain that is (+) leu(+) lac(+) is used to transform a strain that is his leu⁻¹lacc⁻¹. The following percentages of cells are transformed: [Donor],[stra...

Question

03-11-2023
Biology

High School

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DNA from a bacterial strain that is (+) leu(+) lac(+) is used to transform a strain that is his leu⁻¹lacc⁻¹. The following percentages of cells are transformed: [Donor],[stra...

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andrew2604 andrew2604 · Answer 1 · 2025-01-14T01:01:12-05:00

Final answer:

This question pertains to the biological process of bacterial transformation and the techniques used in recombinant DNA technology, where foreign genes are introduced into bacteria to express new proteins.This is a fundamental technique used in genetic engineering to express new genes in a host organism, for example, the lac+ gene in Escherichia coli.

Explanation:

The subject of this question is clearly Biology, specifically focusing on the concepts of transformation in bacteria and recombinant DNA technology, likely covered at the College level in a course on genetics or microbiology. Transformation refers to the introduction of foreign DNA into a bacterial cell, which can come from a plasmid or be facilitated by bacteriophages in the process known as transduction. This is a fundamental technique used in genetic engineering to express new genes in a host organism, for example, the lac+ gene in Escherichia coli. Competent cells that are able to take up DNA do so by various methods, such as exposure to calcium chloride or electroporation. Selectable markers, such as the antibiotic resistance gene, are used to identify cells that have successfully incorporated the recombinant DNA. When cells are grown on media containing the antibiotic, only those with the plasmid containing the resistance gene will survive, easing the identification of transformed cells.