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Answer :
The primary purpose of the Kirby-Bauer disk diffusion test is C) To test the effectiveness of different antibiotics on a bacterial strain.
The Kirby-Bauer test, also known as the disk diffusion test, is a standardized method used to evaluate the sensitivity of bacteria to various antibiotics. This method assesses how well different antibiotics work on a specific bacterial strain by observing the zone of inhibition, which is the clear area around an antibiotic disk where bacteria cannot grow.
Here’s a step-by-step explanation of how the test works:
Preparation: A known bacterial strain is cultured on an agar plate. The bacteria are evenly spread to create a uniform lawn of growth across the entire surface of the agar.
Application of Antibiotic Disks: Small disks impregnated with different antibiotics are placed on the surface of the agar. Each disk contains a known concentration of an antibiotic.
Incubation: The plate is incubated to allow the bacteria to grow. During this time, the antibiotics in the disks diffuse out into the agar.
Evaluation: After incubation, the plate is examined for zones of inhibition. These are the clear circles around the disks where bacteria have been unable to grow due to the presence of the antibiotic.
Interpretation: The size of each zone of inhibition is measured, usually in millimeters. Larger zones indicate that the bacteria are more sensitive to the antibiotic, while smaller zones suggest resistance.
This test is important for determining which antibiotics might be most effective for treating infections caused by the specific bacteria being tested. However, it does not provide information on the minimal inhibitory concentration (MIC) or identify bacterial species, which are assessed by other methods. By allowing healthcare professionals to select effective antibiotics, the Kirby-Bauer test aids in promoting good clinical outcomes in the treatment of bacterial infections.
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