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Answer :
Final answer:
The detector in a UV-VIS spectrophotometer is a PMT or CCD. The wavelength for a calibration curve is chosen based on the λmax of a sample. Quartz is commonly used to make cuvettes due to its transparency to UV light.
Explanation:
In a UV-VIS spectrophotometer, the detector commonly used is usually a Photomultiplier Tube (PMT) or a Charge-Coupled Device (CCD). These detectors are designed to accurately record and measure the properties of light.
The wavelength selected for preparing a calibration curve in spectrophotometry is often chosen based on the sample's absorption maximum, or λmax, which refers to the wavelength where the sample absorbs the most light. This wavelength often coincides with the light's energy that matches the energy gap of the sample's electrons.
The cuvettes used in spectrophotometry are typically made from quartz or sometimes high-quality plastic or glass, depending on the wavelength range. Quartz cuvettes are preferred for UV-VIS spectrophotometry because quartz is transparent to UV light, unlike regular glass.
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