Arrange the following eight statements about the catalytic cycle of chymotrypsin in the correct order:

a. His 57 donates H to Ser 195 O, leading to collapse of the tetrahedral intermediate.
b. Ser 195's nucleophilic O attacks the carbonyl C of the substrate.
c. The C-terminal end of the original substrate diffuses away with a new amino terminus.
d. The N-terminal portion of the original substrate diffuses away with a new carboxylate terminus.
e. Water donates a H to His 57.
f. His 57 catalyzes removal of H from Ser 195 hydroxyl.
g. The hydroxyl OH- attacks the carbonyl of the remaining substrate.
h. His 57 donates H to N of the scissile peptide bond, and the tetrahedral intermediate decomposes.

Choose the correct order:

A. F-H-C-E-B-A-G-D
B. F-A-B-H-G-E-C-D

Question

High School

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Answer :

The correct order of the statements is F-A-B-H-G-E-C-D.

His 57 donates H to Ser 195 O leading to collapse of tetrahedral intermediate
Ser 195's nucleophilic o attacks the carbonyl C of the substrate
The C-terminal end of the original substrate diffuses away with a new amino terminus
The N-terminal portion of the original substrate diffuses away with a new carboxylate terminus
Water donates a H to His 57
His 57 catalyzes removal of H from Ser 195 hydroxyl
The hydroxyl OH- attacks the carbonyl of the remaining substrate
His 57 donates H to N of scissile peptide bond and the tetrahedral intermediate decomposes

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