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Answer :
To make 2.00 mL of a 100 μg/mL BSA solution from a 2.00 mg/mL stock, you will need to dilute the stock solution with a suitable diluent in a specific ratio.
To prepare the desired BSA solution, follow these steps:
1. Calculate the required amount of BSA from the desired concentration:
BSA concentration = 100 μg/mL
BSA volume = 2.00 mL
BSA mass = BSA concentration x BSA volume
= 100 μg/mL x 2.00 mL
= 200 μg
2. Determine the volume of the stock solution needed based on the stock concentration:
BSA concentration (stock) = 2.00 mg/mL
Volume of stock solution = BSA mass / BSA concentration (stock)
= 200 μg / (2.00 mg/mL)
= 0.1 mL or 100 μL
3. Transfer 100 μL of the 2.00 mg/mL BSA stock solution into a container (e.g., a test tube or a volumetric flask).
4. Add a suitable diluent (such as distilled water or an appropriate buffer) to the container to reach a final volume of 2.00 mL. Mix well to ensure proper dilution.
By following these steps, you will obtain 2.00 mL of a 100 μg/mL BSA solution from the 2.00 mg/mL stock solution. It is essential to use precise measuring devices, such as micropipettes, to accurately measure the volumes required. Additionally, use appropriate labware, such as test tubes or volumetric flasks, for preparing and storing the solution.
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