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Answer :
True, a bacterial strain that is pro thi leu− met− will grow on minimal media plus leucine and thiamine. This is because the strain requires exogenous leucine and thiamine for growth, which are provided in the supplemented minimal media.
Leucine and thiamine added to minimum media will not support the growth of a pro-thi leu met bacterial strain. Proline, thiamine, leucine, and methionine can't be synthesised by this strain, which is why it's present. If any of these particular nutrients are lacking when utilising minimal medium, which only contains the nutrients necessary for growth, the strain will not be able to thrive.
Leucine and thiamine are two of the nutrients that the strain lacks, so adding these nutrients to the minimum media won't help the strain develop. Proline, thiamine, leucine, and methionine are only a few of the elements the strain would need in a full medium to maintain its growth.
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