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Generalized transduction is the transfer of a host bacterial chromosome by a phage into another bacterial cell. A donor bacterial strain with the genotype met\(^+\) thr\(^+\) is used to transduce a recipient bacterial strain with the genotype met\(^-\) thr\(^-\). After transduction, the recipient bacterial population is plated on a minimal medium supplemented with methionine.

Which of the following is correct about the bacterial growth in the provided medium above?

A. Bacteria with the met\(^-\) thr\(^+\) genotype will grow in the medium.

B. Non-recombinant recipient bacteria will grow in the medium.

C. All recombinant bacteria will grow in the medium.

D. Bacteria with the met\(^+\) thr\(^-\) genotype will grow in the medium.

Answer :

Final answer:

The recipient bacterial population will grow in the medium supplemented with methionine as they acquire the met+ and thr+ genotype during transduction.

Explanation:

After transduction, the recipient bacterial population will grow in the provided medium supplemented with methionine. The recipient bacterial strain lacks the met- and thr- genes, which are required for the synthesis of methionine and threonine, respectively. However, the donor bacterial strain possesses these genes. During transduction, the phage transfers a random piece of the donor bacterial chromosome, which includes the met+ and thr+ genes, into the recipient bacterial cell.

As a result, the recipient bacterial cells acquire the met+ and thr+ genotype. Therefore, bacteria with the met+ thr+ genotype will be able to grow in the medium supplemented with methionine.

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