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When doing a bacteriophage sensitivity plate, you streak the phage on the plate first, and the bacterial strain second.

A. True
B. False

Answer :

The statement that you should streak the phage on the plate first and the bacterial strain second is false; in practice, the bacterial strain is streaked first to create a lawn, then serial dilutions of the phage are spread on top.

The statement that you streak the phage on the plate first, and the bacterial strain second is false. When performing a bacteriophage sensitivity plate or titering recombinant phage clones, the proper protocol typically involves streaking the bacterial strain to create a confluent layer or lawn on the agar plate first. Subsequently, serial dilutions of the phage lysate are spotted or spread on top of the bacterial lawn. This allows for the observation of clear zones called plaques where the bacteriophage infects and lyses the bacterial cells. These plaques are indicative of phage sensitivity and can be counted to determine the titer of viable virus particles.

Lytic bacteriophages create these clearings by entering a lytic cycle where new phages are produced, leading to the lysis of the host cells and release of the progeny virions into the environment. In contrast, the lysogenic cycle involves the integration of phage DNA into the host genome without immediate destruction of the host cell, which can happen under certain environmental stressors.

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