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Answer :
In bacteriophage sensitivity testing, also known as a plaque assay, the bacterial lawn is established before phage lysate is added, not the other way around. Plaques reveal areas where bacteriophages have lysed the bacterial cells.
The statement 'When doing a bacteriophage sensitivity plate, you streak the phage on the plate first, and the bacterial strain second' is false. When performing a bacteriophage sensitivity or plaque assay, the bacterial lawn is established first. A bacterial lawn is created by plating a high number of bacteria so they grow together, forming a continuous layer on the agar plate. Then, the phage lysate, possibly diluted in a series, is spread on top of this bacterial lawn. The presence of the phage can be observed by the formation of clear zones called plaques, where the bacteriophage has lysed the bacteria.
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