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Answer :
The correct answer is that the fish's eye cells are not visible to the eye or under a dissecting microscope when the fish is frozen.
When Bob placed the frozen fish under the dissecting scope, he would not have been able to see the cells in the fish's eye for several reasons:
1. Resolution Limitations: Dissecting microscopes, also known as stereo microscopes, are designed for low magnification observation of whole specimens or large structures. They typically have a maximum magnification of around 40x to 80x, which is not sufficient to resolve individual cells. Cells are usually on the order of 10 to 100 micrometers in size, and observing them requires a compound microscope with much higher magnification and resolution capabilities, typically starting at 100x and going up to 1000x.
2. Frozen Tissue Integrity: When tissues are frozen, ice crystals can form within the cells, disrupting their structure and making it difficult to observe any cellular details. Additionally, the freezing process can cause the cells to shrink and the tissue to become more opaque, further hindering visibility.
3. Lack of Preparation: To observe cells under a microscope, especially a compound microscope, the tissue usually needs to be prepared in a specific way. This often includes fixation to preserve cell structure, embedding in a medium like paraffin wax, sectioning into thin slices, and staining with dyes to highlight different cellular components. Without these preparatory steps, the cells would not be distinguishable under the microscope.
4. Improper Lighting: Dissecting scopes use incident lighting (light shining down on the specimen from above). This type of lighting may not be suitable for observing the translucent structures of an eye, especially if the specimen is opaque due to being frozen. Transmitted light, which passes through the specimen, is often used for observing thin sections of tissue under a compound microscope.
In summary, Bob's inability to see the cells in the fish's eye through a dissecting microscope when the fish is frozen is due to the microscope's limited resolution, the disruption of cellular structure by freezing, the lack of proper tissue preparation, and the use of incident lighting which is not ideal for observing internal cellular structures. To properly observe the cells, the fish's eye would need to be thawed, properly prepared, and viewed under a compound microscope with the appropriate magnification and staining techniques.
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Answer:
His process is wrong.
Explanation:
Bob must take the eye out of the fish. Then he must cut the eye. After he cuts the eye he needs to take a thin layer of the eye and put it on the glass. A microscope needs light to shine through the specimen. All Bob would see when he put the fish on there would be black. The thick amount of mass is blocking the light.
