High School

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A student formulated a hypothesis that water-soluble pollutants damage living organisms by increasing the permeability of cellular membranes. To test the hypothesis, the student investigated the effect of isopropanol and acetone on beetroot cells. The vacuoles of beetroot cells contain large amounts of betacyanin, a water-soluble pigment that is released into the extracellular environment as a result of increased membrane permeability.

Answer :

Answer:

The absorbance of 460nm light by the treatment solutions was the test performed by the student.

Explanation:

Cell membranes are phospholipid bilayers which are mostly displayed by the 'fluid mosaic model' which consists of proteins, carbohydrate polymers and glycoproteins making them able to move around quite freely amongst the phospholipids. A normal membrane permeability test involves investigating the effects of a named variable on the membrane permeability of any vegetable such as beetroot whose biological name is Beta Vulgaris. The variables to investigate are the influence of solvents and temperature because these factors can change the fluidity of the membrane. Beetroot is an important item for this test because of the distinctive betalains pigment that its stem tuber contains. These pigments form an important signal indicating membrane fluidity as they are usually contained in the vacuole of intact beetroot cells. An instant or constant increase in membrane fluidity will cause the pigment to spill out of the cell, and the amount of pigment can be measured simply by using a colorimeter.

The above description is performed under the test of The absorbance of 460nm light by the treatment solutions.

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Final answer:

Water-soluble pollutants can damage living organisms by increasing the permeability of cellular membranes, as tested with isopropanol and acetone on beet root cells.

Explanation:

Water-soluble pollutants can damage living organisms by increasing the permeability of cellular membranes, which can cause the release of water-soluble pigments. In the case of beet root cells, the vacuoles contain betacyanin, a water-soluble pigment that is released when the membrane permeability increases.



To test this hypothesis, the student investigated the effect of isopropanol and acetone on beet root cells. If the hypothesis is correct, these substances should increase the permeability of the cellular membranes and cause the release of betacyanin.

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