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Answer :
The impact of pooling the e2 and e3 fractions and applying them to a new Ni2+-agarose column on the purity, yield, and activity of rgfp would depend on several factors.
The factors including the composition of the individual fractions, the conditions used for pooling and column chromatography, and the nature of any contaminants or impurities present.
It is difficult to predict happen to the purity, yield, and activity of recombinant green fluorescent protein (rgfp) if the e2 and e3 fractions were pooled and applied onto a new Ni2+-agarose column without additional information.
Purity means the proportion of the target protein (here it is, rgfp) in the final sample. It is relative to other proteins or contaminants that may be present.
Yield means amount of target protein recovered after the purification process.
Activity means the biological or biochemical function of the protein, such as its ability to fluoresce.
The activity of rgfp could affect pooling the fractions and applying them to a new column. If the pooling process causes denaturation or aggregation of rgfp, its activity could be compromised.
If contaminants or impurities in the pooled fractions interfere with the function of rgfp, its activity could also be affected.
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