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If the protein Argonaut in RISC is not functioning, then which kind of posttranscriptional gene regulation is most likely affected?

A. shortening of the microRNAs polyA tail
B. ubiquitination
C. alternative splicing
D. protein phosphorylation
E. inhibition of ribosome assembly
F. RNA interference

Answer :

The most likely affected posttranscriptional gene regulation mechanism when the protein Argonaut in RISC (RNA-induced silencing complex) is not functioning is RNA interference option F is correct answer.

RNA interference (RNAi) is a posttranscriptional gene expression mechanism that involves the degradation or translational repression of target mRNA molecules. It is mediated by small RNA molecules, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), which are incorporated into the RNA-induced silencing complex (RISC). The protein Argonaut plays a crucial role in RISC by binding to small RNAs and guiding them to their target mRNAs.

If the protein Argonaut is not functioning properly, it would impair the assembly and activity of the RISC complex. Consequently, the ability of miRNAs and siRNAs to bind to their target mRNAs and induce gene silencing would be compromised. This would result in a reduced efficiency of RNA interference, leading to deregulation of gene expression at the posttranscriptional level.

Options A, B, C, D, and E are not directly related to the function of Argonaut in RISC and are not typically associated with RNA interference. Therefore, option F, RNA interference, is the most likely affected mechanism in this scenario.

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