Answer :

Final answer:

To breed mares using AI with frozen semen, the semen must be carefully thawed to body temperature, inseminated at the time of ovulation, and the mare must be monitored for pregnancy. Advanced techniques like embryo transfer may also be utilized.

Explanation:

To breed mares using AI with frozen semen, a meticulous and professional procedure must be followed. The process starts with semen thawing, which involves carefully bringing the frozen semen stored in straws back to body temperature, at about 37°C, to maintain sperm viability. Following thawing, timing is critical to ensure the semen is introduced into the mare's reproductive tract synchronously with her ovulation. Veterinary professionals often monitor the mare's cycle to decide the optimal insemination moment.

Artificial insemination itself entails the introduction of the semen into the mare's uterus. The semen is often placed within the uterus using a pipette or tube to maximize the chances of successful fertilization. Post-insemination, the mare is typically monitored for signs of pregnancy. Advanced reproductive techniques might also include identifying the best candidates for embryo transfer, which requires preparing a recipient mare to receive and nurture the developing embryo. The ultimate goal is a safe and successful pregnancy resulting in the birth of a foal.

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