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Answer :
The best statement describes purpose of the streaking technique is c) To decrease the concentration of bacterial cells on the agar surface .
Streaking is a microbiology technique that is used to isolate single colonies of microorganisms from a mixed species or the same species.. This approach is most commonly employed with bacteria, but it is also used with some yeasts.
The streak plate approach is based on the dilution concept. It is referred to as a quick qualitative isolation technique. The basic goal of isolation is to obtain a small number of colonies.
In this procedure, a loop of culture is distributed on an agar plate to separate individual cells sufficiently. The streaking method gradually dilutes the inoculum, allowing bacterial cells to be counted as colony forming units (CFUs).
T-streaking technique is the types of Streak Plate Method.
The T-streaking technique divides a petri dish into three quadrants by creating the letter 'T' on it. It is a discontinuous approach, similar to four-quadrant streaking, in which each quadrant is streaked after loop sterilization, and the quadrant streaked last provides isolated colonies.
Thus, the option c) is correct.
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Final answer:
The purpose of the T-streaking technique is to reduce the risk of contamination on the agar surface by diluting the bacterial cells and spreading them out to obtain pure cultures of bacteria.
Explanation:
The purpose of the T-streaking technique is to reduce the risk of contamination on the agar surface. T-streaking involves using a loop to make a series of streaks on the agar plate, diluting the bacterial cells with each streak. By diluting the cells and spreading them out, it helps ensure that individual colonies can grow without interference from other contaminating cells. This technique is commonly used in microbiology to obtain pure cultures of bacteria.
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