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Answer :
It utilizes the quadrant streak plate method to assess bacterial growth in the presence of antibiotic.
The Kirby-Bauer Test is a laboratory method used to assess the sensitivity of bacterial strains to antibiotics. Among the options provided, option B is not applicable to the Kirby-Bauer Method. Specifically, the Kirby-Bauer Method does not utilize the quadrant streak plate method to assess bacterial growth. Instead, it uses a disk diffusion method where filter paper disks impregnated with antibiotics are placed on an agar plate inoculated with the bacterial strain, and the zones of inhibition around the disks are measured to determine sensitivity.
Key points regarding the Kirby-Bauer Method:
- Uses Mueller-Hinton agar plates for testing.
- Can test several antibiotics on a single culture plate.
- Results are interpreted as sensitive (S), intermediate (I), or resistant (R).
Thus, the correct answer is B.
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E. A - D are all applicable to the Kirby-Bauer Method.
The Kirby-Bauer Method is a laboratory technique used to assess the sensitivity of bacterial strains to antibiotics. It is widely used to determine the most effective antibiotic to use in treating bacterial infections.
The method involves placing paper disks that contain different antibiotics onto a culture plate that has been inoculated with the bacteria of interest. The growth of the bacteria is then assessed to determine the level of sensitivity to each antibiotic.
A, C, and D are all applicable to the Kirby-Bauer Method. The technique involves assessing the sensitivity of bacterial strains to antibiotics, multiple antibiotics can be tested on a single culture plate, and the results of the test can determine whether the bacterial strain is resistant, sensitive, or has intermediate sensitivity.
However, option B is not applicable to the Kirby-Bauer Method. The quadrant streak plate method is not used in this technique. Instead, a sterile swab is used to spread the bacterial culture evenly across the agar surface before the antibiotic disks are placed on the plate. The Kirby-Bauer Method is a simple and effective technique that is widely used in microbiology laboratories for antibiotic susceptibility testing.
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